10on10 Foods high protein atta research banner, showing 25g protein, 14.5g fibre and three ingredients, with wheat flour, rotis and pulses

High Protein Atta: What the Research Supports, and What It Doesn't

10on10 Foods Research Desk

Wheat is short of one amino acid. Jowar was tested at 30%, not 100%. And the khapli study the whole category leans on measured something other than blood sugar.

Where these numbers come from. Every nutrition figure below is either a declared value from our own packs, a result from our published lab reports, or a finding from a named peer-reviewed paper. Where we have not yet published a figure for one of our flours, this page says not published rather than filling the gap with a typical value. We would rather have holes than numbers we cannot show you the source of.

There is a number on the front of every protein atta bag in India right now, and it is doing a great deal of work. Ours says 25g. Another says 17g. A third says 40g. The shopper's reasonable assumption is that a bigger number means more protein doing its job in your body.

It is not that simple, and the reason has been in the nutrition literature for years. Protein is not one substance. It is a string of amino acids, and your body can only build with a protein up to the point where its scarcest essential amino acid runs out. Wheat has a well-documented shortage of one of them.

Here is what three papers actually say, what that means for the flour in your kitchen, and a calculator built only on figures we can show you the source of. We have included the finding that works against one of our own products, because you would find it eventually.

Wheat is short of lysine. That is why there is soya in our atta.

Wheat protein scores 54 out of 100 on the FAO's preferred measure. The amino acid holding it back is lysine.

Protein quality is assessed by DIAAS — the Digestible Indispensable Amino Acid Score, the method the FAO recommends over the older PDCAAS. It scores a protein source by how much of each essential amino acid is actually absorbed, and the final score is set by whichever one falls shortest against human requirements.

Measured against the adult requirement pattern, wheat scores 54%, and the amino acid setting that ceiling is lysine. In the same study, soya flour scored 105% — and critically, soya's own limiting amino acid is not lysine. It is the sulfur amino acids, which is the group wheat happens to have in reasonable supply.

Mathai JK, Liu Y, Stein HH. Values for digestible indispensable amino acid scores (DIAAS) for some dairy and plant proteins may better describe protein quality than values calculated using the concept for protein digestibility-corrected amino acid scores (PDCAAS). British Journal of Nutrition, 2017;117(4):490–499.
DIAAS, adult reference pattern
Bar length is the score. The vertical line marks 100 — the point at which a protein fully meets adult requirements. The label under each bar names the amino acid that set that score.
Soya flour
105
limited by sulfur amino acids
Pea protein
73
limited by sulfur amino acids
Wheat
54
limited by lysine
All three figures: Mathai, Liu & Stein (2017), adult pattern. Wheat and the legumes are limited by different amino acids, which is the entire argument for putting them in the same roti. Indian kitchens have done this for centuries without the vocabulary: roti with dal, khichdi, besan in the batter.

So the useful rule is not buy the biggest number on the front. It is: a wheat flour with a legume milled into it is addressing wheat's actual constraint. Adding more wheat protein does not relieve a lysine shortage. Adding a lysine-rich legume does.

That is why our High Protein Atta is sharbati wheat with soya and chana milled through it, rather than wheat gluten or an isolate stirred in afterwards. Three ingredients, each chosen for what it contributes to the amino acid profile.

What we are not going to claim: we have not had a DIAAS measurement done on the finished blend, so we are not going to print a score for it, and you should be sceptical of anyone who prints one without a lab report behind it. DIAAS is also a score for rating a protein source — it is not a multiplier you apply to grams to get "usable protein", and it cannot be averaged across a blend, because complementation is precisely the effect an average erases. What we can tell you is which constraint the formulation is built to address, and what is declared on the pack.

The jowar trial used 30%, not 100%

Sorghum at 30% of the flour lowered the glucose response — and cut how much people ate at the next meal.

A 2025 randomised crossover trial gave twenty healthy adults pasta made with 30% sorghum flour against a 100% durum wheat control. The red sorghum version produced a significantly lower blood glucose response (−0.35 mmol/l, P = 0.005) and a smaller 120-minute glucose area under the curve (−36.11 mmol/l×min, P = 0.017).

The finding that matters more came afterwards. Participants reported higher satiety (P = 0.029) and lower hunger (P = 0.004), then ate around 794 kJ less at the following meal (P = 0.001) — roughly 190 calories they simply did not eat, hours later, with nobody asking them to restrict anything.

Khan J, et al. Effect of sorghum flour-containing pasta on postprandial glycemia, appetite and energy intake in healthy individuals. European Journal of Clinical Nutrition, 2025. Twenty healthy participants; results are for pasta, not roti.

Read the proportion again: thirty percent. This quietly dissolves the oldest objection to millet flours. Everyone is told to eat more jowar, most people attempt a 100% jowar roti once, fight a dough with no gluten in it, and never try again. The trial suggests the all-or-nothing framing was never necessary.

Two honest caveats. The study was done on pasta, not roti, and the comparison was against durum wheat — so the effect size in your kitchen may differ. And twenty people is a small trial. What it establishes is a direction and a workable proportion, not a guarantee.

What the khapli study actually measured

This is the uncomfortable one. Khapli — emmer wheat, Triticum dicoccum — is sold across India, by us included, with a strong implication about blood sugar. So we read the clinical trial that most of those claims trace back to.

In diabetic patients, khapli wheat significantly improved blood lipids. It did not significantly change blood glucose.

Twenty-two people with diabetes ate 200–300g of flour a day as unleavened bread for six weeks, one group on dicoccum (khapli) wheat, one on ordinary bread wheat.

The lipid results were clear. In the khapli group total lipids fell 14% (p<0.01), triglycerides fell 15% (p<0.01) and LDL cholesterol fell 11% (p<0.05). The control group showed no significant lipid change.

Fasting and postprandial glucose, though, did not move significantly. Fasting glucose drifted downward in the khapli group (132.6 → 112.4 mg/dL), but the change did not reach statistical significance, and neither did the postprandial figures.

Yenagi NB, Hanchinal RR, Patil CS, Koppikar V, Halagi M. Glycemic and lipidemic response to dicoccum wheat in the diet of diabetic patients. International Journal of Diabetes in Developing Countries (RSSDI), 2001. Twenty-two subjects, six weeks.

We are not going to dress that up. If you are buying khapli specifically to bring your blood sugar down, the strongest trial available does not support that claim. It is also a small study from 2001, which cuts both ways — absence of a significant result in twenty-two people is not proof of absence.

What the trial does support is khapli as a lipid story. A 14% fall in total lipids over six weeks, from changing nothing but which flour went into the same rotis, is a real result and a good reason to eat it. Set alongside our declared figure — 14.54g of dietary fibre per 100g, the highest of anything we mill — khapli earns its place on the shelf. Just for a different reason than the one usually advertised.

Dr. Anju recommends

"The families who make real progress are almost never the ones who switched to the most expensive flour. They are the ones who changed what is in the jar — a legume in it for the amino acids, some jowar in it for the glucose curve — and went on eating rotis exactly as before."

"If you change one thing this month, do not change your brand. Put a couple of spoons of besan into the atta you already own and give it four weeks. If your family does not notice the difference on the tawa, that is the change working."

Work out what your household actually eats

This calculator uses only our declared pack values and published lab results. Where we have not published a figure, it says so rather than estimating. To compare against what you currently buy, read the protein figure off your present atta's pack and type it in — that way the comparison uses your number, not our guess about it.

The atta calculator

Protein, fibre and monthly cost for your household — from published figures only.

6
About 180g of atta a day · 5.4kg a month
Roti size
What are you solving for?
Read it off the nutrition panel of the atta you buy now. Leave blank and we simply won't show a comparison.
Our recommendation
Protein / day
Fibre / day
Cost / month
Against the atta you buy now
Grams of protein a day, same number of rotis.
Type the protein figure from your current atta's pack above and we will put the two side by side. We have deliberately not assumed a value for it — ordinary attas vary, and we would rather use yours.
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Every figure here is traceable. Protein and fibre values are our declared pack figures or results from our published lab reports, dated and listed at the bottom of this page. Grams of atta, daily totals and monthly cost are straightforward arithmetic on those figures and our current prices. Nothing on this page is modelled, estimated or adjusted by a factor. Where a flour's fibre has not been lab-published yet, we show that instead of a number.

What we would actually do

If you take one thing from three papers, take this: what is in the jar matters more than the name on it.

If you buy nothing from us today, do this anyway — put a couple of spoons of besan into whatever atta is currently in your kitchen. It is cheap, it addresses the amino acid wheat is genuinely short of, and it is the highest-return change available to a household that already eats rotis every day.

If you would rather not run a blending operation in your kitchen every week, that is exactly what a pre-milled blend is for, and it is what we make.

Sources

  1. Mathai JK, Liu Y, Stein HH. Values for digestible indispensable amino acid scores (DIAAS) for some dairy and plant proteins may better describe protein quality than values calculated using the concept for protein digestibility-corrected amino acid scores (PDCAAS). British Journal of Nutrition, 2017;117(4):490–499. — source for wheat 54%, soya flour 105%, pea protein concentrate 73%, and the limiting amino acid in each.
  2. Khan J, et al. Effect of sorghum flour-containing pasta on postprandial glycemia, appetite and energy intake in healthy individuals. European Journal of Clinical Nutrition, 2025. — source for the 30% sorghum figures, n=20, pasta.
  3. Yenagi NB, Hanchinal RR, Patil CS, Koppikar V, Halagi M. Glycemic and lipidemic response to dicoccum wheat (Triticum dicoccum) in the diet of diabetic patients. International Journal of Diabetes in Developing Countries (RSSDI), 2001. — source for the khapli lipid and glucose results, n=22, six weeks.
  4. FAO. Dietary protein quality evaluation in human nutrition, Food and Nutrition Paper 92 — the report that recommends DIAAS over PDCAAS.
  5. 10on10 Foods third-party lab reports, tested 6 January 2026 — besan (AGSS/FA/26010600013), jowar atta (AGSS/FA/26010600011), ragi atta (AGSS/FA/26010600012). Published at 10on10foods.com/pages/lab-reports.
This article is general nutrition information, not medical advice. The trials cited were run on specific populations, in specific preparations, at small sample sizes, and do not guarantee an individual result. If you are managing diabetes, cholesterol or kidney disease, or you are pregnant, speak to your doctor or a registered dietitian before making your daily staple a variable.
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